Author's response to reviews Title: CD133+ Adult Human Retinal Cells Remain Undifferentiated in Leukemia Inhibitory Factor (LIF) Authors:
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چکیده
Results We demonstrated purification (to 95%) of CD133+ cells from adult human postmortem retina as demonstrated proliferation through BrdU incorporation and expression of the proliferation markers Ki67 and Cyclin D1 was changed to this:We demonstrated purification (to 95%) of CD133 + cells from adult human postmortem retina. Proliferating cells were identified through BrdU incorporation and expression of the proliferation markers Ki67 and Cyclin D1 Conclusion These data support the evidence that CD133 expression characterises a population of cells within the resident adult human retinal which have progenitor cell properties and whose turnover and differentiation is LIF-dependent. Was changed to this:These data support the evidence that CD133 expression characterises a population of cells within the resident adult human retina which have progenitor cell properties and that their turnover and differentiation is influenced by LIF.
منابع مشابه
CD133+ adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor (LIF)
BACKGROUND CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres. METHODS Retinal cell susp...
متن کاملCD 133 + adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor ( LIF ) Debra
Background: CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres. Methods: Retinal cell susp...
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تاریخ انتشار 2008